Introduction: The study was carried out to analyse and give a summary of our experience with the different means of fertility RESERVATION in cancer patients, using conservative methods like injection of Gn-RH. Analoga and antagonists are successful over IVF with subsequent oocyt- and embryo-crypRESERVATION to more invasive ones like laparoscopic ovarian tissue banking for ovarian cryopRESERVATION. We designed a retrospective cohort study. The study setting was Medical School of Vienna, Department of Gynaecological Endocrinology and Reproduction Medicine. 107 patients underwent different kinds of fertility pRESERVATION methods.Materials and Methods: Fertility pRESERVATION counseling is a decision making process for the appropriate method. In case of ovarian tissue, banking describes the intra- and postoperative courses. Main Outcome Measure (s): In case of ovarian tissue making, the operating time, major and minor complications, histological and microbiological results were seen. In case of IVF, the major and minor complications were seen.Results: 85 patients underwent cryopRESERVATION of ovarian tissue, mostly for malignant diseases (78/85, 91.8%). 60 patients (70.6%) underwent laparoscopy for ovarian tissue harvesting only, without any additional surgical procedure. The median operating time was 30 minutes (range: 10- 75 minutes). The intraoperative course was uneventful in these patients. In 2 patients, slight postoperative increases in C-reactive protein levels were found. Microbiological examination revealed no contamination apart from one case revealing sporadic propionibacterium acnes. Histological examination revealed intact ovarian tissue with primordial follicles in 81/85 patients (95.3%). There was only one case of IVF and embryo cryopRESERVATION. 26 patients were treated only with GnRH analoga or antagonist.Conclusion: The increasing life expectancy after chemo- and ionization-therapy brings about new aspects into the life of cancer patients. Maintaining the quality of life would be the new issue and challenge in this group of patients. New methods of ART could be offered for this purpose. One of the most promising new therapy options is OTB, which can be performed by laparoscopy. Laparoscopy is a safe and effective procedure for ovarian tissue harvesting. We suggest microbiological and histological testing of ovarian tissue as mandatory tools to guarantee safety regarding ovarian tissue transplantation.